By Edouard Kurstak
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Extra resources for Arctic and Tropical Arboviruses
1963). , 1970). McLintock (1976) has suggested that early spring Aedes species such as A. campestris, A. spencerii, and A. flavescens may be involved in early summer amplification of WEE virus before C. tarsalis emerges from hibernation. In northern areas where C. tarsalis is not abundant, virus transmission is likely by C. inornata or Aedes mosquitoes (Burton and McLintock, 1970). Arboviruses in Canada B. 43 Eastern Equine Encephalitis Eastern equine encephalitis (EEE) virus was first isolated in Canada in 1938 from the blood of a horse at St.
1972a), Murmansk coast (Gaydamovich et al. A. , 1973). The virus is pathogenic for suckling mice and 2-3-week-old mice upon intracerebral inoculation. The virus replicates in chick embryos and BHK-21 cell cultures without cytopathogenic effect (CPE). Electron-microscopic studies of the virus in both suckling mouse brain cells and tissue cell cultures have revealed crystallike formations and a "loopy net" along with typical virons, about 40 nm in size (Fig. 1 ) . Apparently, these structures are associated with synthesis and formation of virions.
The envelop possesses distinct outlines, and spikes covering the particle can be distinguished in some areas. D. Κ. Lvov, et al. 34 β I I Ο ; 10 15 20 10 I) 20 3 10 Fraction FIGURE 6. Distribution of preparations of Sakhalin virus labeled with Η-uridine in sucrose (A,B) and cesium sulfate (C) density gradients. Preparations in some experiments (B,C) were treated with a 1% solution of the nonionic detergent NP-40. In the inner cavity of the particle, small granules (of about 10 nm) can be observed, which apparently present a cross section of ribonucleoprotein strands.
Arctic and Tropical Arboviruses by Edouard Kurstak