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Lisa Yount's Craig Venter: Dissecting the Genome PDF

By Lisa Yount

ISBN-10: 1604136626

ISBN-13: 9781604136623

ISBN-10: 7814381371

ISBN-13: 9787814381372

This ebook profiles the lifestyles and profession of Craig Venter, who in 1998 introduced that his inner most corporation could map the complete human genetic series.

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Extra info for Craig Venter: Dissecting the Genome

Sample text

Claire Fraser, furthermore, had not received the career advancement that NINDS had promised her, and around 1988, Venter saw a way to help her. The National Institute of Alcohol and Drug Abuse, another branch of NIH, wanted to establish a receptor research laboratory and offered Fraser a chance to head it, so Venter let her take over his laboratory’s receptor work and transfer it to the new location. ” Not being able to work together was a painful change, but they remained close in their personal lives.

Venter insisted that the T in the be capitalized, so that the abbreviation for the institute would be TIGR—pronounced tiger. Craig Venter planned to make sure that before long, all of bioscience would hear his “tiger” roar. indd 35 9/16/11 11:07 AM 3 Spelling Out the Code Craig Venter was thrilled to have what most scientists could only dream of: his own institute, almost unlimited funding for it—Steinberg promised him $10 million to start and another $60 million to be spread over the following 10 years—and a substantial sum in his own pocket.

They used the enzyme reverse transcriptase to make cDNA copies of the messenger RNA molecules that the cells generated as the first step in making various proteins. The researchers broke the long cDNA molecules into smaller pieces with other enzymes, called restriction enzymes. They also broke open ring-shaped pieces of DNA called plasmids, which they would use as vectors, or carriers, for the cDNA. They attached the fragments of cDNA to the plasmid DNA, then “glued” the hybrid plasmid rings back together (ligation).

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Craig Venter: Dissecting the Genome by Lisa Yount


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