By F. Kaneez
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Extra resources for Patch Clamp Technique [neuromed.]
3 Serotonin receptor channel currents in B103 cell (High conductance subset) Exhibits Whole-Cell Current Response of the High B103 Subset to Transient Bath Application of 500 M 5-HT (Figure#14) in Symmetrical Solutions, as 10 M 5-HT was not able to produce any response in this sub set of B103 cells. All serotonin concentrations except 500 M and other drugs were applied to only low and medium subsets of B103 cells. Fig. 21. Channel Subconductance States. 50 ms alteration in the channel conducting state.
Involvement of a novel fast inward sodium current in the invasion capacity of abreast cancer cell line. Biochim Biophys Acta. L. 1974. Clonal cell lines from the rat central nervous system. Nature. 249(454):224-227.  Schubert D. 1975. The uptake of GABA by clonal nerve glia. Brain Research. 84(1):87-98.  Segal M. M and Douglas, A. F. 1997. J. 1994. Distinctive patterns of GABAA receptor subunit mRNAs in 13 cell lines. Journal of Neuroscience. 14(9):5417-5428. , Woodward B. 1975. Membrane potential changes induced by 5hydroxytryptamine in the rabbit superior cervical ganglion.
Further investigation is warranted to clarify this decreased response. D-tubocurarine was employed as a competitive antagonist to identify 5-HT3Rs in B103 celllines. The recorded responses to d-tubocurarine indicated that rather than antagonising 5HT3R activity it was having a modulatory affect on the native B103 receptors for both low and medium subsets. The low cells had a more normal response with a decrease in conductance seen with d-tubocurarine at hyperpolarising potentials, however stimulation with both d-tubocurarine and 10 M 5-HT at hyperpolarising potentials appeared to increase subset conductance to a level above that seen with 10 M 5-HT alone.
Patch Clamp Technique [neuromed.] by F. Kaneez