By Ray Wu; Lawrence Grossman; Kivie Moldave
Recombinant DNA tools are robust, innovative ideas that permit the isolation of unmarried genes in huge quantities from a pool of millions or hundreds of thousands of genes and the amendment of those remoted genes or their regulatory areas for reintroduction into cells for expression on the RNA or protein degrees. those attributes result in the answer of advanced organic difficulties and the creation of latest and higher items within the components of drugs, agriculture, and industry.
Recombinant DNA Methodology, a quantity within the Selected tools in Enzymology sequence produced in benchtop structure, encompasses a number of key articles from Volumes sixty eight, a hundred, one hundred and one, 153, 154, and one hundred fifty five of Methods in Enzymology. the fundamental and customary approaches supplied at a cheap cost should be a useful relief to the graduate scholar and the researcher.
* Enzymes in DNA research
* DNA isolation, hybridization, and cloning
* DNA series analysis
* cDNA cloning
* Gene products
* id of cloned genes and mapping of genes
* tracking cloned gene expression
* Cloning and moving of genes into yeast cells
* Cloning and moving of genes into plant cells
* Cloning and moving of genes into animal cells
* Site-directed mutagenesis
* Protein engineering
* Expression vectors
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Additional resources for Recombinant DNA methodology
Raae, J. R. Lillehaug, and K. Kleppe, Biochemistry 12, 5045 ( 1 973). -W. Knopf, Eur. J. Biochem. 73, 33 (1977). zs and formation of an acid-precipitable enzyme -adenylate complex. 23•25•29 DNA ligases catalyze a variety of inter- and intramolecular joining reactions, and some examples are given in Table I. The reactions cata lyzed by the E. coli enzyme are asterisked. Escherichia coli ligase joins oligo(dT) that is base-paired to poly(dA) ; it also joins oligo(dA) base paired to poly(dT), but this is a much less favorable reaction.
Landy, E. Ruedisueli, L. Robinson, C. Foeller, and W. Ross , Biochemistry 13, 2 1 34 (1974) . H. 0. Smith and G. M. Marley, this series , Vol. 65, p. 104. K. N. Subramanian, S. M. Weissman, B. S. Zain, and R. J. Roberts, J. Mo/. Biol. 110, 297 ( 1 977). J. L. Hines, T. R. Chauncey, and K. L. Agarwal, this series , Vol. 65, p. 153. D. G. Kleid, this series, Vol. 65, p. 163. J. Tomassini, R. Roychoudhury, R . Wu, and R. J. Roberts, Nucleic Acids Res. 5, 4055 ( 1 978). R. E. Gelinas, P. A . Myers, and R.
Fareed, E. M. Wilt, and C. C. Richardson, J. Biol. Chem. 246, 925 ( 197 1). 33 O. Westegaard, D. Brutlag, and A. Kornberg, J. Biol. Chem. 248, 1361 ( 1973). M D. G. Blair, D. B. Clewell, D. J. Sherratt, and D. R. Helinski, Proc. Natl. Acad. Sci. A. 69, 25 1 8 ( 1 972). 00 TABLE I EXAMPLES OF DNA LJGASE REACTIONS a Type of reaction Substrate Template Linkages formed DNA-DNA DNA-DNA DNA-DNA DNA-DNA DNA-DNA DNA-DNA Oligo(dT)* Oligo(dA)* Oligo[d(A-T)J* Oligo(dT) (pdT)11pdC Termini of exonuclease IIIdigested DNA Blunt-ended duplex DNA Poly(dA) Poly(dT) Self-complementary Poly(A) Poly(dA) ?
Recombinant DNA methodology by Ray Wu; Lawrence Grossman; Kivie Moldave