By Barry R. Zirkin, Bernard Robaire (auth.), Erwin Goldberg Ph.D. (eds.)
This quantity includes the lawsuits of the XVth North American Testis Workshop, held in Louisville, Kentucky, April 7 to ten, 1999, to explain present advances in testis biology. the 1st chapters offer an invaluable historic viewpoint of testis body structure and formulate compelling learn questions about very important points of sperm formation and serve as. This prologue units the scene for the rest of the amount that follows a logical development, because the name implies, from stem mobile to mobilephone functionality, yet that's inevitably preceded via intercourse selection, the vital requirement for there being a testis within the first position. this system for this XVth Testis Workshop advanced from recommenda tions of the clinical committee and their recommendation at the number of invited audio system. The XVth Testis Workshop has a robust comparative style with contributions on worms (Caenorhabditis), flies (Drosophila), and chickens, that are versions that let and thereby display the facility of genetic analy ses of the molecular mechanisms concerned about spermatogenesis. A glimpse into the longer term is supplied with details from EST, protein, and genome databases that have already got an influence on growth in our realizing of the male germ telephone. even though there's heavy emphasis in those chapters on mobile and molecular e,¥ents, database mining and translational profiling proper to testis functionality and disorder and assisted copy tech nologies will not be overlooked.
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Extra resources for The Testis: From Stem Cell to Sperm Function
RNA-binding protein, TIAR, is essential for primordial germ cell development. Proc Natl Acad Sci USA 1998;95: 2331-36. 6. Beamer WG, Cunliffe-Beamer TL, Shultz KL, Langley SH, Roderick TH. Juvenile spermatogonial depletion (isd): a genetic defect of germ cell proliferation of male mice. BioI Reprod 1988;38:899-908. 7. Eddy EM, O'Brien DA. Gene expression during mammalian meiosis. Curr Top Dev BioI 1997;37:141-200. 8. Dix DJ, Allen JW, Collins BW, Mori C, Nakamura N, Poorman-Allen P, et ai. Targeted gene disruption of Hsp-70-2 results in failed meiosis, germ cell apoptosis, and male infertility.
Sertoli cell-;-enriched cultures were obtained by subjecting the seminiferous tubule fraction to glycine treatment, followed by sequential collagenase/hyaluronidase digestion and filtration (23). To ascertain the purity of the cellular preparations, the cells were stained for 3bHSD activity and analyzed for expression of cell-type-specific genes (19,24). The Leydig-cell preparations consistently contained fewer than 1 germ cell per high power field «1 %) and appeared homogeneous microscopically.
C 600 OJ" N !! 011 ... 3. Effects of MIS on Leydig cell steroidogenesis. 1% BSA, 100 mg/ml bovine lipoprotein (Sigma), 12 Ilg/ ml gentamycin, and 1 ng/ml LH (NIDDK-oLH-26, National Hormone and Pituitary Program) in a reduced oxygen incubator (5%Co/5% 0) (Nuaire TS Autoflow Co/0 2 incubator) (20,40). The cells were treated with 30 nM MIS or buffer for 48 hours. Conditioned media were collected every 24 hours for steroid analysis. On the third day, 22R-hydroxycholesterol was added as a testosterone substrate for the last 6 hours.
The Testis: From Stem Cell to Sperm Function by Barry R. Zirkin, Bernard Robaire (auth.), Erwin Goldberg Ph.D. (eds.)